| Título: | LC-MS metabolomics from study design to data-analysis – using a versatile pathogen as a test case |
| Autores: |
Berg, Maya; Institute of Tropical Medicine Antwerp Vanaerschot, Manu; Institute of Tropical Medicine Antwerp Jankevics, Andris; University of Glasgow and University of Groningen Cuypers, Bart; Institute of Tropical Medicine Antwerp Breitling, Rainer; University of Manchester Dujardin, Jean-Claude; Institute of Tropical Medicine Antwerp |
| Fecha: | 2013-02-06 |
| Publicador: | Computacional and structural biotechnology journal |
| Fuente: |
 |
| Tipo: |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article |
| Tema: | No aplica |
| Descripción: | Thanks to significant improvements in LC-MS technology, metabolomics is increasingly used as a tool to discriminate the responses of organisms to various stimuli or drugs. In this minireview we discuss all aspects of the LC-MS metabolomics pipeline, using a complex and versatile model organism, Leishmania donovani, as an illustrative example. The benefits of a hyphenated mass spectrometry platform and a detailed overview of the entire experimental pipeline from sampling, sample storage and sample list set-up to LC-MS measurements and the generation of meaningful results with state-of-the-art data-analysis software will be thoroughly discussed. Finally, we also highlight important pitfalls in the processing of LC-MS data and comment on the benefits of implementing metabolomics in a systems biology approach. |
| Idioma: | Inglés |
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