| Título: | Rational approaches for engineering carbon-carbon bond forming enzymes |
| Autores: |
Baker, Perrin Seah, Stephen; University of Guelph |
| Fecha: | 2012-10-02 |
| Publicador: | Computacional and structural biotechnology journal |
| Fuente: |
 |
| Tipo: |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article |
| Tema: | No aplica |
| Descripción: | Enzymes that catalyze carbon-carbon bond formation can be exploited as biocatalyst for synthetic organic chemistry. However, natural enzymes frequently do not possess the required properties or specificities to catalyze industrially useful transformations. This mini-review describes recent work using knowledge-guided site-specific mutagenesis of key active site residues to alter substrate specificity, stereospecificity and reaction specificity of these enzymes. In addition, examples of de novo designed enzymes that catalyze C-C bond reactions not found in nature will be discussed. |
| Idioma: | Inglés |
1 Systems biology and metabolic engineering of Arthrospira cell factories por Klanchui, Amornpan,Vorapreeda, Tayvich,Vongsangnak, Wanwipa,Kannapho, Chiraphan,Cheevadhanarak, Supapon,Meechai, Asawin | 6 The Role of INDY in Metabolic Regulation por Willmes, Diana M; Charité University School of Medicine Berlin,Birkenfeld, Andreas L; Charité University School of Medicine Berlin |
2 Structure-based Methods for Computational Protein Functional Site Prediction por KC, Dukka B; North Carolina A&T State University | 7 The Biochemistry of Vitreoscilla hemoglobin por Stark, Benjamin C.; Illinois Institute of Technology,Dikshit, Kanak L.; Institute of Microbial Technology,Pagilla, Krishna R.; Illinois Institute of Technology |
3 Computer-Aided Protein Directed Evolution: a Review of Web Servers, Databases and other Computational Tools for Protein Engineering por Verma, Rajni; Jacobs University Bremen,Schwaneberg, Ulrich; RWTH Aachen University,Roccatano, Danilo; Jacobs University Bremen | 8 A method to predict edge strands in beta-sheets from protein sequences por Guilloux, Antonin,Caudron, Bernard,Jestin, Jean-Luc |
4 MD simulation studies to investigate iso-energetic conformational behaviour of modified nucleosides m2G and m22G present in tRNA por Bavi, Rohit S,Sambhare, Susmit B,Sonawane, Kailas D; Structural Bioinformatics Unit,
Department of Biochemistry,
Shivaji University, Kolhapur 416 004,
Maharashtra (M.S.), India. | 9 Conformational Sampling in Template-Free Protein Loop Structure Modeling: An Overview por Li, Yaohang; Old Dominion University |
5 Metabolomics in the identification of biomarkers of dietary intake por O’Gorman, Aoife,Gibbons, Helena,Brennan, Lorraine | 10 Development of microorganisms for cellulose-biofuel consolidated bioprocessings: metabolic engineers’ tricks por Mazzoli, Roberto; University of Torino, Italy. |