| Título: | Angiotensin-converting enzyme and p22phox polymorphisms and the risk of coronary artery disease in a low-risk Spanish population |
| Autores: |
Mata Balaguer, Trinidad Herrán, Roberto de la Ruiz Rejón, Carmelo Ruiz Rejón, Manuel Garrido Ramos, Manuel A. Ruiz Rejón, Fernando |
| Fecha: |
2011-12-14 2011-12-14 2004-06 |
| Publicador: | RUA Docencia |
| Fuente: |
 |
| Tipo: | info:eu-repo/semantics/article |
| Tema: |
Coronary heart disease Angiotensin-converting enzyme Subunit alpha of the cytochrome b558 Linkage disequilibrium Genética |
| Descripción: |
Objective: To evaluate the genetic contribution to myocardial infarction in a homogeneous Caucasian population (a Mediterranean Spanish population) with very low frequency of coronary heart disease (CHD). Design: We analyzed a total of 210 subjects, younger than 55 years, considered to be a low-risk population (104 cases of myocardial infarction and 106 control), and genotyped them (using polymerase chain reaction and sequencing) for the angiotensin-converting enzyme (ACE) insertion/deletion (ACE I/D) and for the C242T polymorphism of NADPH oxidase p22phox. Also, we sequenced 23 alleles of the ACE gene (9 D and 14 I) for the region that includes the end of the intron 16 and the exon 17. Results: The ACE genotype-prevalence values for II, ID and DD were 4.81%, 28.85% and 66.34%, respectively, among the myocardial infarction patients, and 2.83%, 71.70% and 25.47% among controls. The statistical analysis comparing patients and controls revealed significant differences (χ2=25.09, P=0.00000055) between the two subpopulations. Also, we found a strong association between the genotype DD and the risk of suffering CHD (odds ratio (OR): 3.64; 95% CI: 2.37–8.07). The prevalence of the CC, TC and TT genotypes of p22phox gene among healthy controls proved to be 53.77%, 44.34% and 1.89%, while those of myocardial infarction were 58.65%, 39.42% and 1.93%, respectively. The association of C242T polymorphism of the p22phox gene with CHD was not statistically significant, (χ2=0.49, P=0.48). Logistic-regression analysis demonstrated that the independent risk factor for developing myocardial infarction was the DD genotype of ACE gene. Finally, our results indicate that alleles I and D of ACE gene are differentiated at three positions (nucleotide sites 14,480, 14,488 and 14,521) of which, the positions 14,480 and 14,488 were in absolute linkage disequilibrium. Conclusions: Among subjects of a Mediterranean population with low risk for CHD, the presence of DD ACE genotype could be a risk factor for myocardial infarction, and we confirm the linkage disequilibrium between two nucleotide positions of the ACE gene and the polymorphism for an Alu insertion. This work has been financed by Spanish grants of Fondo de Investigaciones Sanitarias (FIS 94/0553) and by Plan Andaluz de Investigación (Research Group CVI0200). |
| Idioma: | Inglés |
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