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Título: Human adolescent nephronophthisis: Gene locus synteny with polycystic kidney disease in pcy mice
Autores: Omran, Heymut
Villaquirán, Aminta
Fargier Delgado, Bernardo
Häffner, Karsten
Burth, Suse
Fernandez, Carmen
Nothwang, Hans-Gerd
Schnittger, Susanne
Lehrach, Hans
Woo, David
Brandis, Matthias
Sudbrak, Ralf
Hildebrandt, Friedhelm
Fecha: 2005-11-18
2005-11-18
2005-11-18
2001-01-01
2005-11-18
Publicador: Universidad de los Andes Venezuela
Fuente:
Tipo:
Tema: Escuela de Medicina
Facultad de Medicina
Medicina y Salud
Artículos
Descripción: Human adolescent nephronophthisis: Gene locus synteny with polycystic kidney disease in pcy mice Omran, Heymut; Häffner, Karsten; Burth, Suse; Fernandez, Carmen; Fargier, Bernardo; Villaquiran, Aminta; Nothwang, Hans-Gerd; Schnittger, Susanne; Lehrach, Hans; Woo, David; Brandis, Matthias; Sudbrak, Ralf and Hildebrandt, Friedhelm Abstract In a large Venezuelan kindred, a new type of nephronophthisis was recently identified: Adolescent nephronophthisis (NPH3) is a late-onset recessive renal cystic disorder of the nephronophthisis/medullary cystic group of diseases causing end-stage renal disease at a median age of 19 yr. With the use of a homozygosity mapping strategy, the gene (NPHP3) was previously localized to chromosome 3q22 within a critical interval of 2.4 cM. In the current study, the NPHP3 genetic region was cloned and seven genes, eight expressed sequence-tagged sites, and seven microsatellites were physically localized within the critical disease interval. By humanmouse synteny analysis based on expressed genes, synteny between the human NPHP3 locus on chromosome 3q and the pcy locus on mouse chromosome 9 was clearly demonstrated, thus providing the first evidence of synteny between a human and a spontaneous murine renal cystic disease. By fluorescence in situ hybridization the chromosomal assignment of NPHP3 to chromosome 3q21-q22 was refined. Renal pathology in NPH3 was found to consist of tubular basement membranes changes, tubular atrophy and dilation, and sclerosing tubulointerstitial nephropathy. This pathology clearly resembled findings observed in the recessive pcy mouse model of late-onset polycystic kidney disease. In analogy to pcy, renal cyst development at the corticomedullary junction was found to be an early sign of the disease. Through cloning of the NPH3 critical region and mapping of expressed genes, synteny between human NPH3 and murine pcy was established, thus generating the hypothesis that both diseases are caused by recessive mutations of homologous genes. Artículo Publicado en: Journal of the American Society of Nephrology. J Am Soc Nephrol 12: 107-113, 2001 Copyright © 2001 by the American Society of Nephrology.
aminta@ula.ve
bernardofargier@hotmail.com
Nivel monográfico
Idioma: Español