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Título: Expression of measles fusion protein in insect and human cells using Eukaryotic expression vectors
Autores: Marshall, Philip.
Fecha: 1988
Publicador: McGill University - MCGILL
Fuente:
Tipo: Electronic Thesis or Dissertation
Tema: Biology, Cell.
Biology, Microbiology.
Descripción: Measles virus is an animal enveloped virus that is a member of the genus morbillivirus in the paramyxoviridae family. Its envelope contains two surface glycoproteins H and F which are required for viral attachment and entry respectively. Virus penetration occurs via a process which involves fusion of the viral membrane with the plasma membrane at the cell surface. Replication of the virus thus follows and leads to giant cell (syncytia) formation.
The infectivity of measles virus is dependent upon a host proteolytic cleavage of the F$ sb0$ glycoprotein into two active subunits F$ sb1$ and F$ sb2$. This cleavage was later shown to expose a hydrophobic sequence at a NH$ sb2$ terminal of the F$ sb1$ which is directly involved in cell fusion and virus penetration.
In order to increase our knowledge concerning cell mediated fusion events we have expressed the fusion glycoprotein of measles virus in insect and human cells by using recombinant baculo- and adenoviruses respectively. Analysis by SDS-PAGE demonstrated that our protein was first synthesized as a 60 Kd protein and cleaved subsequently into its two respective subunits F$ sb1$ and F$ sb2$ of 40 Kd and 20 Kd respectively. Hemolysis assays confirmed the biological activity of this protein in both systems. However, the fusion protein was unable to fuse insect cells.
Idioma: en