| Título: | Fine mapping of the nuclear restorer locus for cytoplasmic male sterility in Brassica napus |
| Autores: | Stollar, Rachel. |
| Fecha: | 2001 |
| Publicador: | McGill University - MCGILL |
| Fuente: |
 |
| Tipo: | Electronic Thesis or Dissertation |
| Tema: |
Cytoplasmic male sterility. Rape (Plant) -- Cytogenetics. |
| Descripción: |
This thesis will discuss the 'Polima' cytoplasmic male sterility (CMS) system (pol) in Brassica napus (Canola) and detailed genetic mapping of the region surrounding restorer gene ( Rfp) for that system This fine mapping of the Rfp region will facilitate efforts to clone the gene that will eventually lead to its characterization. Knowledge of the structure of Rfp will provide insight in the molecular mechanisms governing mitochondrial gene statement as well as pollen production and may lead to the development of alternative methods of pollination control. In addition, it is possible that nuclear restorer genes for other CMS systems in other crops may be similar to that of the 'Polima' system. Map based cloning requires the identification of DNA markers tightly linked to Rfp. Two PCR based markers which are located on either side of Rfp were developed. These markers allowed facile screening of a large population. RFLP markers used in this study are based on the synteny between B. napus and the well known crucifer A. thaliana. (Abstract shortened by UMI.) |
| Idioma: | en |
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