| Título: | Developing novel lipases from Bacillus thermocatenulatus to improve enantioselectivity by directed evolution |
| Autores: | Liu, Andrew Man Fai, 1975- |
| Fecha: | 1999 |
| Publicador: | McGill University - MCGILL |
| Fuente: |
 |
| Tipo: | Electronic Thesis or Dissertation |
| Tema: | Chemistry, Organic. |
| Descripción: |
In order to make use of enzymes in unnatural applications more efficiently, novel enzymes have to be developed and they may arise from an existing enzyme with similar performance. The fast way to improve the performance of the enzyme is by directed evolution. It alters a naturally existing protein, without in depth knowledge of the starting enzyme and produces mutants containing the required activities. The lipase from Bacillus thermocatenulatus is an alko-thermophilic enzyme. Optimization of the lipase production is carried out for use with the new screening method using a pH indicator to evaluate hydrolytic activities. This newly cloned lipase is next characterized with two substrate libraries made up of commercially available general and enantiomerically pure esters. An important chiral synthon solketal is identified as the directed evolution target molecule for this lipase. By modifying with error-prone PCR, the enantioselectivity of the lipase towards solketal n-octanoate is improved. |
| Idioma: | en |
1 Investigations on the form-genera Beauveria and Tritirachium por MacLeod, Donald Murdock | 6 Treatment and recovery in first-episode psychosis : a qualitative analysis of client experiences por Windell, Deborah L. |
2 Seismic sensitivity of tall guyed telecommunication towers. por Ghodrati Amiri, Gholamreza. | 7 |
3 Exploring the Relationship Between Assets and Family Stress Among Low-Income Families por Rothwell, David W.,Han, Chang-Keun | 8 |
4 The case for asset-based interventions with indigenous peoples: Evidence from Hawai‘i por Rothwell, David W. | 9 |
5 Second Thoughts: Who Almost Participates in an IDA Program? por Rothwell, David W.,Han, Chang-Keun | 10 Recent contributions to the phenomenology of musical time : a critical survey por Beaudreau, Pierre |