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Título: Nrg1p and Rfg1p in Candida albicans yeast-to-hyphae transition
Autores: Lacroix, Céline.
Fecha: 2008
Publicador: McGill University - MCGILL
Fuente:
Tipo: Electronic Thesis or Dissertation
Tema: Candida albicans -- growth & development.
Morphogenesis.
Hyphae -- metabolism.
Fungal Proteins.
Repressor Proteins.
Saccharomyces cerevisiae Proteins.
Oligonucleotide Array Sequence Analysis -- methods.
Descripción: The ability of Candida albicans to change morphology plays several roles in its virulence and as a human commensal. The yeast-to-hyphae transition is tightly regulated by several sets of activating and repressing pathways. The DNA-binding proteins Rfg1p, Nrg1p and the global repressor Tup1p are part of the repressors found to regulate this morphogenesis. Knowledge of these repressors is based on extrapolations from homology to S. cerevisiae and from expression studies of mutants in inducing conditions, all of which are indirect means of determining a protein's function. We proposed a genome-wide location study of the Nrg1 and Rfg1 transcription factors to obtain direct data to identify their in vivo targets. Our results suggest different avenues for Nrg1p function and a regulation behaviour diverging from the previously suggested model: Nrg1p acts not only as a repressor but also as a transcription activator. Furthermore it regulates its target genes through binding in their coding regions instead binding to the expected regulatory elements on promoters.
Idioma: en