| Título: | Design and assembly of a multimodal nonlinear laser scanning microscope |
| Autores: | Bélisle, Jonathan. |
| Fecha: | 2006 |
| Publicador: | McGill University - MCGILL |
| Fuente: |
 |
| Tipo: | Electronic Thesis or Dissertation |
| Tema: |
Scanning electron microscopes -- Design and construction. Scanning electron microscopy. Fluorescence microscopy. Three-dimensional imaging. |
| Descripción: | The objective of this thesis is to present the fabrication of a multiphoton microscope and the underlying theory responsible for its proper functioning. A basic introduction to nonlinear optics will give the necessary knowledge to the reader to understand the optical effects involved. Femtosecond laser pulses will be presented and characterized. Each part of the microscope, their integration and the design of the microscope will be discussed. The basic concepts of laser scanning microscopy are also required to explain the design of the scanning optics. Fast scanning problems and their solutions are also briefly viewed. As a working proof, the first images taken with the microscope will be presented. Fluorescent beads, rat tail tendon, gold nanoparticles and pollen grain images using various nonlinear effects will be shown and discussed. |
| Idioma: | en |
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