| Título: | Study of the erythropoiesis activity of nano-encapsulated forms of erythropoietin |
| Autores: |
Khasenbekova, Zhanagul; Center for Life Sciences, Nazarbaev University, Astana Gulayev, Alexandr; Center for Life Sciences, Nazarbaev University, Astana Nechayeva, Elena; The State Research Center of Virology and Biotechnology VECTOR, Novosibirsk Nurgozhin, Talgat; Center for Life Sciences, Nazarbaev University, Astana Zhumadilov, Zhaxybay; Center for Life Sciences, Nazarbaev University, Astana |
| Fecha: | 2014-01-24 |
| Publicador: | Central Asian Journal of Global Hearth |
| Fuente: |
 |
| Tipo: | |
| Tema: | No aplica |
| Descripción: | Introduction: The recombinant human erythropoietin (rhEPO) is used in the treatment of anemia. In order to improve its pharmacokinetic properties, nanoparticles of biodegradable polymers of natural or synthetic origin were used. The aim of this study was to investigate the effect of new nano-encapsulated forms of recombinant human erythropoietin for oral use on the erythropoiesis in the cyclophosphamide immunosuppression model. Material and methods: The CHOpE immortalized cells culture (a primary producer of rhEPO "Vector" in Russia) was used. The following biodegradable polymers were chosen: 0.05% and 0.005% carbopol, 0.05% and 0.005% kollidon, and 0.05% and 0.005% pectin. Immunosuppression was obtained by a single dose of i.p. injection of cyclophosphamide (250 mg/kg) in white mice (18-20 g). During the next 5 days, the nano-encapsulated erythropoietin (100 ED/mouse) was administered orally to each mouse. After 5 and 10 days, the cell count of the number of blood reticulocytes and the myelogram of bone marrow were performed. The control group of mice received injections of Eprex. Results: On the 5th day of the experiment, the highest level of reticulocyte was observed in the samples of erythropoietin with kollidon (0.05%) and pectin (0.005%) nanoparticles. On the 10th day, the highest activity was observed in the samples of erythropoietin substance with pectin at 0.05% and 0.005% concentrations. The levels of reticulocytes in these groups reached 13.53% and 14.55%, respectively. The results of the myelogram during immunosuppression showed some activity of erythropoietin in conjunction with both concentrations of pectin when a two-fold increase in the number of erythroblasts was observed on the 5th day. High degrees of erythrokaryocytes in the state of mitosis were observed in the 0.05% pectin samples. Similar results were observed in equivalent groups of control animals on the 10th day of the experiment, which is compatible with the data on Eprex action. Conclusion: The erythropoietic activity of nano-encapsulated forms of erythropoietin was observed in the 0.05% and 0.005% pectin samples in the cyclophosphamide immunosuppression model setting. |
| Idioma: | Inglés |
1 The Central Asian Journal of Global Health: A Supercourse Journal por Linkov, Faina; University of Pittsburgh,LaPorte, Ronald; University of Pittsburgh | 6 The HUPO Human Proteome Project (HPP), a Global Health Research Collaboration por Omenn, Gilbert S.; University of Michigan |
2 New directions in building a scientific social network: Experiences in the Supercourse project and application to Central Asia por Hennon, Meredith; University of Pittsburgh,LaPorte, Ronald E.; University of Pittsburgh,Shubnikov, Eugene; Institute of Internal Medicine, Novosibirsk, Russia,Linkov, Faina; University of Pittsburgh | 7 Keeping Up with the Times por Burambayeva, Valentina |
3 Modernization and growth in Kazakhstan por Sharman, Almaz; Nazarbayev University | 8 Public Health Challenges and Priorities for Kazakhstan por Aringazina, Altyn; Kazakhstan School of Public Health, Almaty, Republic of Kazakhstan,Gulis, Gabriel; University of Southern Denmark, Esbjerg, Denmark,Allegrante, John P.; Columbia University |
4 Missing Millions and Measuring Progress towards the Millenium Development Goals with a focus on Central Asian States por Carr-Hill, Roy; Centre for Health Economics,
University of York
YORK, YO10 5DD
UK | 9 Effects of Amyloid Beta Peptide on Neurovascular Cells por Askarova, Sholpan; Center for Life Sciences, Nazarbaev University,Tsoy, Andrey; Department of Biodiversity and Bioresources
71 Al-Farabi av. Biological Faculty Building
Al-Farabi Kazakh National University,Shalakhmetova, Tamara; Al-Farabi Kazakh National University, Biology Faculty, Almaty,Lee, James C-M; Department of Biological Engineering
240 Agricultural Engineering Building
University of Missouri - Columbia |
5 The Case of NRENs in Central Asia por Janz, Robert Franciscus; Centre for Information Technology,
University of Groningen,Kutanov, Askar; Academician, National Academy of Sciences, Kyrgyz Republic | 10 Is the Supercourse useful for Latin America? por Padilla-Raygoza, Nicolas; University of Guanajuato,Linkov, Faina; Division of Cancer Prevention and Population Science, University of Pittsburgh Cancer Institute,Shubnikov, Eugene; Institute of Internal Medicine, Novosibirsk, Russian Federation,LaPorte, Ronald E.; Department of Epidemiology, Graduate School of Public Health, University of Pittsburgh Cancer Institute,Diaz-Guerrero, Rosalina; Departrment of Nursing and Obstetrics, Division of Health Sciences and Engineering, Campus Celaya Salvatierra, University of Guanajuato |