| Título: | Hydrogen-rich saline ameliorates the retina against light-induced damage in rats |
| Autores: |
Tian, Liang Zhang, Lei Xia, Feng An, Jing Sugita, Yashino Zhang, Zuoming |
| Fecha: | 2013-09-01 |
| Publicador: | BioMed Central Ltd. |
| Fuente: |
Ver documento |
| Tipo: | Research |
| Tema: | Light damage, Hydrogen, Retina |
| Descripción: | Abstract Oxidative reactions are thought to be a major cause of light-induced retinal degeneration. This study was designed to investigate the effects of hydrogen-rich saline (HRS) on the prevention and treatment of light-induced retinal injury in rats. Male Sprague–Dawley rats were divided randomly into three groups: light damage, HRS prevention (5 ml/kg, 30 min before intensive light exposure), and HRS treatment (5 ml/kg per day for 5 days, after intensive light exposure), respectively. The right eye of each rat was exposed to 5000 lux constant white light-emitting diode (LED) light for 3 h, and the left eye was covered to serve as the blank control. Electroretinograms were recorded 5 days later, and the thickness of the outer nuclear layer (ONL) was measured after hematoxylin and eosin (H&E) staining. The results showed that the electroretinogram b-wave amplitudes and the mean ONL thicknesses of rats were significantly greater in the HRS prevention (P < 0.001) and treatment (P < 0.001) groups than in the light damage. These results indicated that peritoneal injection of HRS provides protection and treatment against light-induced retinal degeneration in rats. |
| Idioma: | Inglés |