| Título: | Gene expression analysis of the proteinase inhibitor gene PiA of potato (Solanum tuberosum) by reverse transcription polymerase chain reaction (RT-PCR) |
| Autores: | Zhang, Tieling, 1968- |
| Fecha: | 2002 |
| Publicador: | McGill University - MCGILL |
| Fuente: |
 |
| Tipo: | Electronic Thesis or Dissertation |
| Tema: |
Protease inhibitors. Potatoes -- Genetics. |
| Descripción: | In this study the gene expression pattern of a novel proteinase inhibitor II (PI-II) gene, PiA, in potato (Solanum tuberosum ), was analyzed using RT-PCR. The PiA gene was found to have enhanced expression in expanding tubers of a greenhouse-grown diploid clone (DC), different stages of 'Russet Burbank' tubers and expanding microtubers of in vitro-grown 'Russet Burbank' and 'Bintje'. PiA mRNA was more abundant in the tuber vascular ring and perimedullary zone areas than in other tuber tissues of DC. The PiA gene was also expressed in flower buds/flowers, shoot apices, leaf blades, stems and roots of 'Russet Burbank'. The PiA mRNA levels did not show significant change after IAA treatment in detached leaf blades and tubers of 'Russet Burbank'. Results suggest that the PiA gene is constitutively expressed and could be involved in tuber developmental processes. To the best of our knowledge, PiA is the first reported PI-II gene expressed in most tissues/organs of potato plants. |
| Idioma: | en |
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