Título: Estimation of lignin in red clover forage : application of trisodium periodate oxidation.
Autores: Pazur, John Howard.
Fecha: 1946
Publicador: McGill University - MCGILL
Fuente:
Tipo: Electronic Thesis or Dissertation
Tema: Agricultural Chemistry.
Descripción: The indefinite knowledge of the constitution and interrelation of the cell wall components has led to the development of highly empirical methods for their estimation. After the removal of the soluble constituents from finely ground plant material with suitable inert solvents, the residue consists almost entirely of cellulose, hemicellulose and lignin. Many of the chemical and physical properties of some of these constituents have not been definitely established. The methods most commonly employed for the estimation of the lignin fraction are based on the principle of insolubility of lignin in strong mineral acids at room temperatures. It has been known for some time that the determination of lignin by these methods is accompanied by many difficulties and uncertainties. Empirical conditions have been established for the removal of the polysaccharide compounds from the lignin fraction. In the case of materials high in nitrogenous compounds, considerable difficulty, which has not yet been completely overcome, has been encountered in the removal of all the nitrogen from the lignin fraction. [...] A study of the lignin fraction of dried and undried clover samples of varying degrees of maturity is reported. The lignin values were obtained by both direct and difference methods of analysis. The effect of periodic acid oxidation of the plant material on the amount and nature of the lignin fractions isolated is also reported.
Idioma: en