A computer vision system which tracks and analyses living cells and their offspring from sequential two-dimensional images is described. The cells are segmented using a method which combines global thresholding, component labeling and noise filtering. The shapes of the cells…

Clathrin-mediated membrane budding mainly occurs at the cell surface, where it drives the formation of endocytic vesicles necessary for the internalization of membrane proteins, and at the trans-Golgi network (TGN), where it mediates the trafficking of cargo proteins from the…