| Título: | RESPONSE SURFACE METHODOLOGY FOR THE OPTIMIZED PRODUCTION OF AN ALKALOPHILIC LACCASE FROM GAMMA-PROTEOBACTERIUM JB |
| Autores: |
Singh, Gursharan Ahuja, Naveen Sharma, Prince Capalash, Neena |
| Fecha: | 2009-01-30 |
| Publicador: | Bioresources |
| Fuente: |
 |
| Tipo: |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| Tema: | Alkalophilic laccase; Production; RSM; Gamma-proteobacterium JB |
| Descripción: | Gamma-proteobacterium JB, an alkali-tolerant soil isolate, produced laccase (8X103 nkat/L) in M162 medium. The optimization of process conditions (pH, incubation time, agitation, and CuSO4 concentration) for laccase production during submerged fermentation was carried out using response surface methodology (RSM) based on a central composite design (CCD). Maximum laccase production achieved was 7.4 X 104 nkat/L at pH 8.0, 210 rpm, 100 µM of CuSO4 after 60 h of incubation. This design of experiment methodology increased laccase production by 9.3 fold over the control. Experimental findings were in close agreement with the model predictions. |
| Idioma: | Inglés |