Descripción: |
The activities of primary ammonia assimilation
enzymes were analyzed in isolated placentas of habanero
peppers (Capsicum chinense Jacq.). The placentas were
cultured in vitro and exposed to conditions promoting
capsaicinoid accumulation, such as treatments with salicylic
acid (SA) and methyl jasmonate (MeJa). Although
exposure to both inducers resulted in increased accumulation
of capsaicinoids, the induction by SA was more pronounced.
Glutamine synthetase (GS) activity, which
incorporates ammonia into glutamine, increased more than
six fold under such conditions, suggesting GS participation
in fulfilling the demand for amino acids required to support
the increase in capsaicinoid synthesis. Glutamate dehydrogenase
(GDH), which has been involved in nitrogen
assimilation in non-photosynthetic tissues such as placentas,
was apparently not involved; its activity decreased
in tissues exposed to the inducers. Thus, under the conditions
tested, the activation of secondary metabolism
required activation of basal nitrogen metabolism. |