| Título: | Regulation of Adenine Nucleotide Concentration at Endothelium-Fluid Interface by Viscous Shear Flow |
| Autores: |
Shen, Jian Gimbrone, Michael A. Jr. Luscinskas, Francis W. Dewey, C. Forbes Jr. |
| Fecha: |
2005-08-31 2005-08-31 1993 |
| Publicador: | MIT |
| Fuente: |
 |
| Tipo: | Article |
| Tema: |
adenine viscous shear flow |
| Descripción: |
The action of adenine nucleotides on vascular endothelial cells is apparently mediated by the local flow conditions. Because
nucleotides are sequentially degraded from ATP - ADP - AMP -- adenosine by ecto-enzymes at the endothelial surface, it has been
hypothesized that the observed flow effect is caused by the flow-dependent change of nucleotide concentration at the cell surface. In this study, we have calculated the concentration profiles of adenine nucleotides at the cell surface under flow conditions encountered in
an in vitro parallel-plate flow system, as has been used in several related experimental studies. When medium containing uniformly
distributed ATP is perfused over endothelial monolayers, our results show that ATP concentration in the cell vicinity gradually decreases
in the streamwise direction as a result of enzymatic degradation. This hydrolysis of ATP results in the generation of ADP, and ADP
concentration in turn gradually increases at the cell surface. The concentration profiles of nucleotides are dependent on the levels of
applied wall shear rate. As the corresponding shear stress increases from 0.1 to 30 dynes/cm^2, ATP concentration at the cell surface at
the center of coverslip increases from 0.66 to 0.93. Under no-flow conditions, our model predicts a steady decline of ATP concentration
and a transient increase of ATP-derived ADP, comparable to the published results of previous experiments. These numerical results,
combined with our recent experimental data, provide insights into the cellular mechanisms by which hemodynamic flow modulates the
effects of vasoactive agents on endothelium. Biophysical Journal, 1993 |
| Idioma: | Inglés |
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