| Título: | Effects of vanadate and potassium depletion on renal H, K-ATPase protein expression |
| Autores: |
Somchit Eiam-Ong Ratanaporn Jerawatana Wipawee Kittikowit Rapeeporn Mannontarat Thaweesak Tirawatnapong Somchai Eiam-Ong |
| Fecha: | 2010-02-11 |
| Publicador: | Asian Biomed |
| Fuente: |
Ver documento |
| Tipo: | Peer-reviewed Article |
| Tema: | No aplica |
| Descripción: | p class="MsoNormal"span style="font-size: 11.0pt; font-family: quot;Microsoft Sans Serifquot;,quot;sans-serifquot;; color: #003300;"Background: Vanadate (V) inhibits while potassium (K) depletion stimulates collecting tubule H, K-ATPase activity. In the presence of V, K depletion could not restore the decreased H,K-ATPase activity. The effects of V and K depletion on renal H,K-ATPase protein expression might explain such observation./span/pp class="MsoNormal"span style="font-size: 11.0pt; font-family: quot;Microsoft Sans Serifquot;,quot;sans-serifquot;; color: #003300;"Objective: To examine the effects of V and K depletion on renal H,K-ATPase protein expression. /span/pp class="MsoNormal"span style="font-size: 11.0pt; font-family: quot;Microsoft Sans Serifquot;,quot;sans-serifquot;; color: #003300;"Methods: Rats treated with normal saline solution (NSS) or V (5 mg/kg body weight) received either normal potassium (NK) or low potassium (LK) diet for 10 days. Protein expressions of renal H,K-ATPase alpha;/spanspan style="font-size: 11.0pt; font-family: quot;Cambria Mathquot;,quot;serifquot;; mso-bidi-font-family: quot;Cambria Mathquot;; color: #003300;"â‚/spanspan style="font-size: 11.0pt; font-family: quot;Microsoft Sans Serifquot;,quot;sans-serifquot;; color: #003300;" and alpha;/spanspan style="font-size: 11.0pt; font-family: quot;Cambria Mathquot;,quot;serifquot;; mso-bidi-font-family: quot;Cambria Mathquot;; color: #003300;"â‚‚/spanspan style="font-size: 11.0pt; font-family: quot;Microsoft Sans Serifquot;,quot;sans-serifquot;; color: #003300;" isoforms were determined by immunohistochemistry./span/pp class="MsoNormal"span style="font-size: 11.0pt; font-family: quot;Microsoft Sans Serifquot;,quot;sans-serifquot;; color: #003300;"Results: Both NK and LK animals treated with V had significantly increased vanadium levels in serum, urine, and renal tissues. LK diet caused hypokalemia. Animals treated with LK and V showed progressive hypokalemia. LK stimulated renal H,K-ATPase alpha;/spanspan style="font-size: 11.0pt; font-family: quot;Cambria Mathquot;,quot;serifquot;; mso-bidi-font-family: quot;Cambria Mathquot;; color: #003300;"â‚/spanspan style="font-size: 11.0pt; font-family: quot;Microsoft Sans Serifquot;,quot;sans-serifquot;; color: #003300;" protein expression in both cortex and medulla but enhanced H,K-ATPase alpha;/spanspan style="font-size: 11.0pt; font-family: quot;Cambria Mathquot;,quot;serifquot;; mso-bidi-font-family: quot;Cambria Mathquot;; color: #003300;"â‚‚/spanspan style="font-size: 11.0pt; font-family: quot;Microsoft Sans Serifquot;,quot;sans-serifquot;; color: #003300;" protein expression only in the cortex. Vanadate did not affect H,K-ATPase alpha;/spanspan style="font-size: 11.0pt; font-family: quot;Cambria Mathquot;,quot;serifquot;; mso-bidi-font-family: quot;Cambria Mathquot;; color: #003300;"â‚/spanspan style="font-size: 11.0pt; font-family: quot;Microsoft Sans Serifquot;,quot;sans-serifquot;; color: #003300;" protein expression in both NK and LK groups. Vanadate unaltered H,K-ATPase alpha;/spanspan style="font-size: 11.0pt; font-family: quot;Cambria Mathquot;,quot;serifquot;; mso-bidi-font-family: quot;Cambria Mathquot;; color: #003300;"â‚‚/spanspan style="font-size: 11.0pt; font-family: quot;Microsoft Sans Serifquot;,quot;sans-serifquot;; color: #003300;" protein expression in NK animals but could attenuate the increased expression in LK group./span/pspan style="font-size: 11.0pt; font-family: quot;Microsoft Sans Serifquot;,quot;sans-serifquot;; mso-fareast-font-family: quot;Times New Romanquot;; color: #003300; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: TH;"Conclusion: The magnitude of direct inhibitory effect of V on renal H,K-ATPase activity with small suppressive effect on protein expression is greater than the stimulatory effect of K depletion on H,K-ATPase activity and protein expression./span |
| Idioma: | No aplica |