| Título: | Increased myocardial energy reservation by creatine supplementation and estrogen replacement in exercise-trained ovariectomized hamsters |
| Autores: |
Kedsara Rakpongsiri Suwanakiet Sawangkoon |
| Fecha: | 2010-01-02 |
| Publicador: | Asian Biomed |
| Fuente: |
Ver documento |
| Tipo: | Peer-reviewed Article |
| Tema: | No aplica |
| Descripción: | Background: Menopause in elder women may induce various dysfunctions in the cardiovascular system. Previous studies show that creatine (Cr) supplementation, estrogen (Eâ‚‚) replacement, or exercise training are effective for cardiac performance. Objective: To examine the combined effect of Cr and/or Eâ‚‚ with exercise training on myocardial energy reservation, using ovariectomized female hamsters. Materials and methods: Female Golden Syrian hamsters were used for this study. A hundred ovariectomized hamsters were divided into no-exercise and exercise-trained groups, in which each group was separated into control (n=10), Cr depletion (Cr-; n=10), Cr supplementation (Cr+; n=10), Eâ‚‚ replacement (Eâ‚‚; n=10) and Cr supplementation combined with Eâ‚‚ replacement (Cr+Eâ‚‚; n=10). In the exercise-trained group, wheel-running exercise (10 min. a day, 5 days a week) was imposed for 9 weeks, and the exercise metabolic rate (EMR) was measured. After the animals were sacrificed, the right ventricles were cut and weighed, which were rapidly frozen in liquid nitrogen for storage at -70°C. The contents of Cr, phosphocreatine (PCr), total Cr (TCr) and creatine kinase (CK) activities were measured, using the spectrophotometric method. Creatine transporter protein (CrT) was measured by Western blotting. Results: Cr+ or Eâ‚‚ combined with exercise training yielded greater accumulation of all myocardial metabolic phosphate contents, CK activity and CrT protein than Cr+ or Eâ‚‚ alone. All parameters regulating myocardial Cr metabolism improved greatly in Cr+ plus Eâ‚‚ combined with exercise training. Conclusion: Creatine supplementation and estrogen replacement, when combined with exercise training, become more effective for myocardial energy reservation via in estrogen-deficient hamsters. |
| Idioma: | No aplica |