| Título: | EXPRESSION OF ras GENE IN EXPERIMENTAL HEPATOCARCINOGENESIS IN TREE SHREWS |
| Autores: |
Ban, Ke-chen Su, Jian-jia Yang, Chun Qin, Liu-liang Li, Yuan Huang, Guo-hua Luo, Xiao-ling Duan, Xiao-xian Yan, Rui-qi |
| Fecha: | 2013-03-11 |
| Publicador: | Chinese Journal of Cancer Research |
| Fuente: |
Ver documento |
| Tipo: | |
| Tema: | No aplica |
| Descripción: | Objective: In order to investigate the relationship between the expression of ras gene and the development of hepatocellular carcinoma (HCC). Materials and Methods: The experimental tree shrews were divided into four groups: group A, infected human hepatitis B virus (HBV) and exposed to aflatoxin B1 (AFB1); group B, infected human HBV alone; group C, only exposed to AFB1; group D, use as controls. The serial bioptic liver tissues were detected for ras p21 protein using immunohistochemical method. Results: The total p21 protein positive rates in group A, B, C and D were 35.3%, 5.3%, 13.3%, 0, respectively, thus the significant difference were showed between group A and group B (P<0.05); The HCC incidences in group A, B, C and D were 47.1%, 0, 13.3%, 0, respectively, and there was a significant difference between group A and C (P<0.05). The incidences of HCC in the animals with and without p21 protein positive in group A were 100% and 18.2%, respectively, and there was a significant difference among them (P<0.01). Conclusion: HBV and AFB1 play a remarkable synerg_istic role in the development of HCC; they can enhance the expression of ras gene. The over-expression of ras gene is closely related to pathogenesis of HCC in tree shrews. |
| Idioma: | Inglés |