Título: | Neutrophil apoptosis : a marker of disease severity in sepsis and sepsis-induced acute respiratory distress syndrome |
Autores: |
Fialkow, Léa Bozzetti, Mary Clarisse Fochesatto Filho, Luciano Rodrigues Filho, Edison Moraes Ladniuk, Roberta Maboni Milani, Adriana Rosa Pierozan, Paula Prolla, João Carlos Downey, Gregory P. Moura, Rafael Moraes de Vachon, Eric |
Fecha: |
2010-05-08 2006 |
Publicador: | |
Fuente: |
Ver documento |
Tipo: |
Artigo de periódico Estrangeiro |
Tema: |
Apoptose Neutrófilos |
Descripción: | Introduction Apoptosis of neutrophils (polymorphonuclear neutrophils [PMNs]) may limit inflammatory injury in sepsis and acute respiratory distress syndrome (ARDS), but the relationship between the severity of sepsis and extent of PMN apoptosis and the effect of superimposed ARDS is unknown. The objective of this study was to correlate neutrophil apoptosis with the severity of sepsis and sepsis-induced ARDS. Methods A prospective cohort study was conducted in intensive care units of three tertiary hospitals in Porto Alegre, southern Brazil. Fifty-seven patients with sepsis (uncomplicated sepsis, septic shock, and sepsis-induced ARDS) and 64 controls were enrolled. Venous peripheral blood was collected from patients with sepsis within 24 hours of diagnosis. All surgical groups, including controls, had their blood drawn 24 hours after surgery. Control patients on mechanical ventilation had blood collected within 24 hours of initiation of mechanical ventilation. Healthy controls were blood donors. Neutrophils were isolated, and incubated ex vivo, and apoptosis was determined by light microscopy on cytospun preparations. The differences among groups were assessed by analysis of variance with Tukeys. Results In medical patients, the mean percentage of neutrophil apoptosis (± standard error of the mean [SEM]) was lower in sepsis-induced ARDS (28% ± 3.3%; n = 9) when compared with uncomplicated sepsis (57% ± 3.2%; n = 8; p < 0.001), mechanical ventilation without infection, sepsis, or ARDS (53% ± 3.0%; n = 11; p < 0.001) and healthy controls (69% ± 1.1%; n = 33; p < 0.001) but did not differ from septic shock (38% ± 3.7%; n = 12; p = 0.13). In surgical patients with sepsis, the percentage of neutrophil apoptosis was lower for all groups when compared with surgical controls (52% ± 3.6%; n = 11; p < 0.001). Conclusion In medical patients with sepsis, neutrophil apoptosis is inversely proportional to the severity of sepsis and thus may be a marker of the severity of sepsis in this population. |
Idioma: | Inglés |
1 | 6 |
2 A vida em separado : estudo de um condomínio fechado de Porto Alegre por Quadros, Caroline Souza de | 7 |
3 | 8 O clítico acusativo na redação escolar por Dutra, Líria Romero |
4 "O passado está prenhe do futuro" : a escrita da história no Instituto Histórico e Geográfico do Rio Grande do Sul (1920-30) por Silveira, Daniela Oliveira | 9 Superfície de imagens : o tema do olhar em Borges e em Cortázar por Botelho, José Francisco Hillal Tavares de Junqueira |
5 | 10 |