1. Inicio
  2. Atrás
L
Título: Metabolic engineering of plant alkaloid biosynthesis
Autores: Sato, Fumihiko
Hashimoto, Takashi
Hachiya, Akira
Tamura, Ken-ichi
Choi, Kum-Boo
Morishige, Takashi
Fujimoto, Hideki
Yamada, Yasuyuki
Fecha: 2001-01-02
2000-12-26
Publicador: The National Academy of Sciences
Fuente: Ver documento
Ver documento
Tipo: Text
Tema: Biological Sciences
Descripción: Plant alkaloids, one of the largest groups of natural products, provide many pharmacologically active compounds. Several genes in the biosynthetic pathways for scopolamine, nicotine, and berberine have been cloned, making the metabolic engineering of these alkaloids possible. Expression of two branching-point enzymes was engineered: putrescine N-methyltransferase (PMT) in transgenic plants of Atropa belladonna and Nicotiana sylvestris and (S)-scoulerine 9-O-methyltransferase (SMT) in cultured cells of Coptis japonica and Eschscholzia californica. Overexpression of PMT increased the nicotine content in N. sylvestris, whereas suppression of endogenous PMT activity severely decreased the nicotine content and induced abnormal morphologies. Ectopic expression of SMT caused the accumulation of benzylisoquinoline alkaloids in E. californica. The prospects and limitations of engineering plant alkaloid metabolism are discussed.
Idioma: en
Artículos similares:
1 
Comparison of written reports of mammography, sonography and magnetic resonance mammography for preoperative evaluation of breast lesions, with special emphasis on magnetic resonance mammography por Malur, Sabine,Wurdinger, Susanne,Moritz, Andreas,Michels, Wolfgang,Schneider, Achim
6 
The Million Women Study: design and characteristics of the study population por
2 
BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite por Bernard-Gallon, Dominique J,Déchelotte, Pierre,Vissac, Cécile,Aunoble, Bénédicte,Cravello, Laetitia,Malpuech, Georges,Bignon, Yves-Jean
7 
Glutathione S-transferase M1 null genotype: lack of association with tumour characteristics and survival in advanced breast cancer por Lizard-Nacol, Sarab,Coudert, Bruno,Colosetti, Pascal,Riedinger, Jean-Marc,Fargeot, Pierre,Brunet-Lecomte, Patrick
3 
Transforming growth factors-β are not good biomarkers of chemopreventive efficacy in a preclinical breast cancer model system por Zujewski, JoAnne,Vaughn-Cooke, Anika,Flanders, Kathleen C,Eckhaus, Michael A,Lubet, Ronald A,Wakefield, Lalage M
8 
Cyclin D1 expression during rat mammary tumor development and its potential role in the resistance of the Copenhagen rat por Korkola, James E,Wood, Geoffrey A,Archer, Michael C
4 
Smoking and high-risk mammographic parenchymal patterns: a case-control study por Sala, Evis,Warren, Ruth,McCann, Jenny,Duffy, Stephen,Luben , Robert,Day, Nicholas
9 
Increased cell survival by inhibition of BRCA1 using an antisense approach in an estrogen responsive ovarian carcinoma cell line por Annab, Lois A,Hawkins, Rebecca E,Solomon, Greg,Barrett, J Carl,Afshari, Cynthia A
5 
Altered expression of estrogen receptor-α variant messenger RNAs between adjacent normal breast and breast tumor tissues por Leygue, Etienne,Dotzlaw, Helmut,Watson, Peter H,Murphy, Leigh C
10 
A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland por Gordon, Katrina E,Binas, Bert,Chapman, Rachel S,Kurian, Kathreena M,Clarkson, Richard W E,John Clark, A,Birgitte Lane, E,Watson, Christine J