| Título: | Diabetes Results in Structural Alteration of Chondroitin Sulfate in the Urine |
| Autores: |
Zhao, Tingting; Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China. Lu, Xiaoguang; Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China. Davies, Neal M; Faculty of Pharmacy, University of Manitoba, Winnipeg, Canada. Gong, Yuewen; Faculty of Pharmacy, University of Manitoba, Winnipeg, Canada. Guo, Jingzhen; Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China. Zhang, Haojun; Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China. Li, Zhiguo; Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China. Hong, Jing; Department of Endocrinology, China-Japan Friendship Hospital, Beijing, China. Fu, Guixiang; Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China. Li, Ping; Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, China. |
| Fecha: | 2013-07-30 |
| Publicador: |
Journal of Pharmacy & Pharmaceutical Sciences Journal of Pharmacy & Pharmaceutical Sciences |
| Fuente: |
Ver documento |
| Tipo: |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| Tema: | No aplica |
| Descripción: | Purpose. The assessment of the clinical significance of chondroitin sulfate in patients with type 2 diabetes mellitus (T2DM) and diabetic nephropathy (DN) for the detection of the relationship between chondroitin sulfate (CS) structure and disease. Methods. Healthy control (n=15), type 2 diabetic patients with normalbuminuria (n=12), and patients with microalbuminuria (n=13) were enrolled in the study. Total sulfated glycosaminoglycans (GAGs) concentration in the first morning urine was evaluated by 1,9-dimethylmethylene blue method and the composition was determined by agarose gel electrophoresis. Urinary chondroitin sulfate was quantified by a combination of treatment with specific lyase digestions and separation of products by SAX-HPLC. Results: GAGs concentration significantly increased in diabetic patients with microalbuminuria compared to diabetic patients with normalbuminuria. Qualitative analysis of urinary GAGs revealed the presence of chondroitin sulfate, heparan sulfate, and low-sulphated chondroitin sulphate-protein complex (LSC-PG). There was a decrease in CS and an increase in LSC-PG in the urine of patients with diabetes compared to healthy controls. Moreover, in diabetic patients, chondroitin sulfate contains more 6-sulfated disaccharide and less 4-sulfated disaccharide. There was a statistically significant difference in ratio of 6-sulfated disaccharide to 4-sulfated disaccharide among the three groups. Conclusions: GAGs were significantly increased in diabetic patients with microalbuminuria. The levels of urinary GAGs, ratio of LSC-PG/CS, as well as ratio of 6-sulfated to 4-sulfated disaccharides could be useful markers for diagnosis of patients with diabetic nephropathy. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page. |
| Idioma: | Inglés |
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