| Título: | Genetic relationship between roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions through optimization of PCR based RAPD method |
| Autores: |
Omal saad A. K. M. Aminul Islam; Associate Professor, Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh Murshida Akhter Jahan Mohamad Osman |
| Fecha: | 2013-06-04 |
| Publicador: | United Arab Emirates University |
| Fuente: |
Ver documento |
| Tipo: | |
| Tema: | DNA extraction; RAPD-PCR optimization; genetic similarity; phylogenetics |
| Descripción: | The PCR based RAPD analysis was used to study genetic relationship among roselle and kenaf accessions. Extraction genomic DNA samples of nine roselle (Hibiscus sabdariffa L.) and kenaf (Hibiscus cannabinus L.) accessions were successfully performed by Cetyl Trimethyl Ammonium Bromide (CTAB) method. RAPD analysis using two chloroplast primers and one mitochondrial primer produced about 62 bands of different sizes. Most of the fragments were polymorphic where 2-3 fragments were monomorphic in each primer. Dendogram generated using RAPD polymorphism classified sixteen accessions into two main groups A and B. Five kenaf accessions included in group A whereas nine roselle accessions and two kenaf accessions were included in second group B. Roselle accessions were forming a group with a range of similarity coefficients of 0.5333 to 0.8780. Bengkalis was close to roselle accessions although it was from different Hibiscus sp. Noonsoon was very close to roselle accessions having a range of jaccard similarity coefficients of 0.5000 to 0.6326. UKMR 1 and UKMR 2 were genetically closer to each other with jaccad similarity coefficient of 0.8780. On the other hand, V36 and Thainung showed the highest similarity coefficient (0.7878) among kenaf accessions in group A. This study has successfully differentiated between two species by using chloroplast and mitochondrial markers which was due to high level of polymorphism. However, the chloroplast primers were more effective than M13 to study the genetic variation between roselle and kenaf. |
| Idioma: | Inglés |