| Título: | Lesion scoring and P450 Isoenzyme activity in liver of hepatocarcinogenesis rats treated with Strobilanthes Crispus |
| Autores: |
Hanachi, P Fauziah, O Asmah, R |
| Fecha: | 2012-04-24 |
| Publicador: | Shahid Beheshti University of Medical Sciences |
| Fuente: |
Ver documento |
| Tipo: |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| Tema: | No aplica |
| Descripción: | AbstractObjective: In this study, we have undertaken efforts to ascertain the anticancer potency of S. crispus (SC) extract on diethylnitrosamine (DEN) and acetylaminofluorene (AAF) induced HCC with special attention to hepatic drug metabolism and to investigate the effect of SC on preneoplastic marker enzyme activity specifically of microsomal aniline hydroxylase (AH) activity and lesion scoring in rats treated with DEN and AAF and controls.Materials and Methods: Thirty male Sprague Dawley rats were divided to six equal number groups. In the first three groups, hepatocellular carcinoma was induced with diethylnitrosamine and acetylaminofluorene. Three groups in each branch were randomly assigned to receive 5% w/v of SC extract, glycyrrhizin or no treatment. After 12 weeks of treatment, the rats were sacrificed. Lesion scoring analysis and Aniline hydroxylase assays were performed as outcome measures.Results: The obtained results have shown a significant, increase (p<0.05) of liver microsome AH in cancer group rats after 12 weeks. Treatment with glycyrrhizin caused decrease in liver AH activity compared to control group. Meanwhile, treatment with SC caused overall decrease in liver AH activity almost near to control group. Meanwhile, microscopic observation of the lesion score during hepatocarcinogenesis revealed that cells of cancer group without treatment were severely necrotic at week 12. S. crispus treatment reduced the severity in cancer group rats at week 12. Conclusion: S. crispus only ameliorated the cancer incidence in the liver, however did not fully recover the liver tumor similar to the normal cells. This might be due to short experimental duration. The chemopreventive action may be due to the scavenging of the reactive oxygen radicals from the system, as well as inhibition of the enzymes responsible for the activation of DEN. In this study, SC extract may act as a chemopreventive agent which exerts its protective effects by inhibition of enzymes involved in metabolic activation of carcinogen (phase I enzyme i.e ANH)Keywords: Strobilanthes crispus, hepatocarcinogenesis, lesion score and aniline hydroxylase 1. Premalatha B, Sachdanandam P. Potency of Semocarpus Anacarddium. Linn nut milk extract against aflatoxin B1-induced hepatocarcinogenesis Reflection on microsomal biotransformation enzymes. Pharmacological Research. 2000; 42(2): 161-166.2. Lewis W.H., Elvin-Lewis, M.P.F. From Plants affecting man’s Health : Medical Botany. In Cancer. Edited by Wiley, A. New York: Interscience publication. 1997; 105-148.3. Rates, S.M.K. Plants as source of drugs. Toxicon 2001; 39: 603-613.4. Sunatro P.A. Materia Medika Indonesia. Jakarta: Penerbit Ditektoral Pengawasan Obat dan Makanan ; 1977.5. Hanachi P, Shamaan NA Ramli J, Arshad JH,. Syed MA.. The effect of Benzo(a)pyrene on glutathione s-transferase, glutathione peroxidase in the liver and kidney mouse mus musculus. Pakistan Journal Medical Science. 2003; 19: 197-202.6. Tepsuwan A., Kupradinum P., Kusamran W.R. : Effect of Siamese Cassia leaves on the activities of chemical carcinogen metabolizing enzymes and on mammary gland carcinogenesis in the rat. Mutation Research: 1999; 428 : 363-373.7. Conney A.H, Wang Z.Y, Huang M.T, Ho C.T,Yang C.S. (1992): Inhibitory effect of oral administration of green tea on tumourigenesis by ultraviolet light, 12-0-tetradecanoylphorbol-13-acetate and n-nitrosodiethylamine in mice. In Cancer Chemoprevention. Edited by W. Lee, L. martin, B.W. Charles and K. Gary. New York : CRC Press Inc. ; 1992 : 362 8. Elizabeth Manickam. The nutritional and antinutritional composition of Strobilanthes crispus (L) Bremek and its anticancer effect during hepatocarcinogenesis. Master Thesis, University Putra Malaysia. 1999.9. Solt D, Farber E. New principle for the analysis of chemical carcinogenesis. Nature 1976; 263: 701-703.10. Hesham M. S, Mostafa H. M, O'Connor P.J, Rafferty J.A. , Michael J. D. Evidence for the presence of active cytochrome P450 systems in Schistosoma mansoni and Schistosoma haematobium adult worms. FEBS Letters 2002; 519(1-3): 205-209.11. Stevens A., Lowe J.S, Young B. From Staging and grading of chronic hepatitis: In Wheater’s Basic Histopathology: Toronto; 2002: 157.12. Imai Y, Ito A, Sato R. Evidence for biochemically different types of vesicles in the hepatic microsomal fraction. J Biochem. (Tokyo) 1966, 60: pp 417.13. Waxman D.J, Morrissey J.J, Leblanc G.A. Female-Predominant rat hepatic P450 forms j (IIEI) and 3 (IIAI) are under hormonal regulatory controls distinct form those of the sex-specific P450 forms. Endocrinol. 1984; 124: 2954-2966.14. Bradford M.M.A: Rapid and sensitive method for quantitation of microgram quantities of protein utilising the principle of protein dye binding. Anal. Biochem 1976; 50: 249-253.15. Jeena K.J, JoyK.C, Kuttan R. Effect of Emblica officinalis, Phyllanthus amarus and Picrorrhiza kurroa on N-nitrosodiethylamine induced hepatocarcinogenesis. Cancer Letters 1999; 136: 11-16. |
| Idioma: | Inglés |
1 Health and Education - 2 por Akbari, Mohammad Esmaeil | 6 Effectiveness of Mindfulness-Based Cognitive Therapy on Reduction of Depression and Anxiety Symptoms in Mothers of Children With Cancer por Mehranfar, Mojtaba; Dept. of Counselling, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran,Younesi, Jalal; Dept. of Counselling, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran,Banihashem, Abdollah; Dept. of Internal Medicine and Paediatrics, University of
Medical Sciences, Mashhad, Iran |
2 The Study of DNA Methylation of bax Gene Promoter in Breast and Colorectal Carcinoma Cell Lines por Alipour, Mohsen; Dept. of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran,Zargar, Seyed Jalal; Dept. of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran,Safarian, Shahrokh; Dept. of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran,Fouladdel, Shamile; Dept. of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran,Azizi, Ebrahim; Dept. of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran,Jafargholizadeh, Naser; Dept. of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran | 7 Prevention and Management of Mucositis in Patients With Cancer: a Review Article por [wlia, ّatemeh; Dept. of Oral Medicine, Yazd Shahid Sadoughi University of Medical Sciences, Yazd, Iran,«azemeini, ُeidkazem; Burn hospital, Yazd Shahid Sadoughi University of Medical Sciences, Yazd, Iran,gholami, neda; Dept. of Oral Medicine, Yazd Shahid Sadoughi University of Medical Sciences, Yazd, Iran |
3 Cancer Prevention; Cancer Risk Management por Akbari, Mohammad Esmaeil | 8 Psychosocial Care for Breast Cancer: Physicians' Perspective por Kaviani, Ahmad; Department of surgery, Tehran University of Medical Sciences, Tehran, Iran,Mehrdad, N; Iranian Center for Breast Cancer (ICBC), Academic Center for Education, Culture and Research (ACECR), Tehran, Iran,Yunesian, M; school of Public Health , Environmental Research Center, Tehran University of Medical Sciences, Tehran, Iran,Shakiba, B; Students’ Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran,Ebrahimi, M; Iranian Center for Breast Cancer (ICBC), Academic Center for Education, Culture and Research (ACECR), Tehran, Iran,Majidzadeh, K; Iranian Center for Breast Cancer (ICBC), Academic Center for Education, Culture and Research (ACECR), Tehran, Iran,Akbari, ME; Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran |
4 Euthanasia in Cancer Patients, Islamic Point of View por Mousavi, SM; Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran,Akbari, A; Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran,Akbari, ME; Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran,Najd Sepas, H; Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran | 9 Cell Survival Entropy and Cellular Resistance Activation Dose: Effect of Calprotectin on Gastric Adenocarcinoma Cell Line por Rezaei-Tavirani, Mostafa,Rahmati-Roodsari, Mohammad,Mirzaie, Mehdi,Amini Geram, Pooneh,Sobhi, Sara |
5 Evaluation of Prognostic Factors In Patients with Metastatic Breast Cancer por Saeidi Saedi, H,Seilanian Toosi, M,Mirsadraei, M,Roodbari, S | 10 Antiphospholipid Antibodies in Neoplastic Patients por Mortazavizadeh, MR,Sadeghmanesh, R |