| Título: | Impact of Phospho-ELK-1 Expression in Enterocytes inBiodosimetry after Low-dose Irradiation |
| Autores: |
Österreicher, J.; University of Defense, Trebešská 1575, 500 01 Hradec Králové, Czech Republic Driak, D.; University and Teaching Hospital Na Bulovce Budínova 2, 180 81 Prague, Czech Republic Vilasova, Z.; University of Defense, Trebešská 1575, 500 01 Hradec Králové, Czech Republic Vavrova, J.; University of Defense, Trebešská 1575, 500 01 Hradec Králové, Czech Republic |
| Fecha: | 2007-11-01 |
| Publicador: | Defence Scientific Information & Documentation Centre |
| Fuente: |
Ver documento |
| Tipo: | |
| Tema: |
Radiation, biodosimetry, phospho-ELK-1, image analysis, immunohistrochemistry, biomarkers Biomedical Sciences |
| Descripción: | Previously the higher phospho-ELK-1 (p-ELK-1) expression in enterocytes 24-96 h aftersub-lethal irradiation was measured. Therefore, purpose of this study is to examine in vivoexpression of this common ERK1/2 and JNK/SAPK target during the first hours after low-doseirradiation to assess ability of this transcriptional factor as a biodosimetric marker after the singlewhole-body irradiation. The laboratory rats were randomly divided into 12 groups and irradiatedwith whole-body gamma radiation dose of 0.25 Gy, 0.5 Gy, and 1.0 Gy. Samples of jejunum weretaken 1 h, 2 h, 4 h, and 24 h later, the p-ELK-1 was immunohistochemically detected and thenits expression was measured by computer image analysis. A significantly increased expressionof p-ELK-1 in enterocytes was measured 1 h, 2 h, and 4 h after 0.5 Gy and 1.0 Gy irradiation,24 h after all radiation doses, respectively. Peak of p-ELK-1 expression was observed at 0.5 Gyat all time points after irradiation. The detection of p-ELK-1 might be considered as a perspectivequalitative in vivo biodosimetric marker for military purposes expressed by proliferative cells24 h after irradiation with doses from 0.25 Gy to 1.0 Gy. |
| Idioma: | Inglés |
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