| Título: |
In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium - doi: 10.4025/actasciagron.v34i4.15143 |
| Autores: |
Brondani, Gilvano Ebling; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP) Araujo, Marla Alessandra; Universidade Federal de Lavras Alcântara, Berenice Kussumoto; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP) Carvalho, Janice Guedes; Universidade Federal de Lavras Gonçalves, Antonio Natal; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP) Almeida, Marcilio de; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP) |
| Fecha: | 2012-09-06 |
| Publicador: | Acta Scientiarum. Agronomy |
| Fuente: |
 |
| Tipo: |
Pesquisa com fatores controlados |
| Tema: |
5.01.03.04-0 bud induction; callogenesis; tissue culture; mineral nutrition 5.01.03.04-0 bud induction; callogenesis; tissue culture; mineral nutrition |
| Descripción: |
The in vitro organogenesis of woody species plays an essential role in the improvement of forest products by providing saplings with high commercial value. Furthermore, mineral nutrition plays an important role in the induction of organogenic responses. The objective of this study was to evaluate the effects of boron and calcium in the organogenesis of nodal segments from seedlings of Eucalyptus grandis growing under in vitro conditions. The concentration of boron and calcium in MS medium was modified to induce organogenic responses in 45-day-old nodal segments used as explants. After 60 days, the fresh weight, dry weight, ratio of fresh and dry weight, relative water content and relative matter content accumulated by the explants were evaluated. The concentrations of boron and calcium in the culture medium influenced the in vitro organogenic control of Eucalyptus grandis. Reduced combinations of boron and calcium induced callus formation and dry matter accumulation in the explants. A boron concentration of 100% (1.10 mg L-1) combined with 100% (119.950 mg L-1) and 200% (239.900 mg L-1) of calcium, and 200% (2.20 mg L-1) of boron combined with 100% (119.950 mg L-1) of calcium allowed the induction of well-developed buds, which can be used for the regeneration of micro-plants. The in vitro organogenesis of woody species plays an essential role in the improvement of forest products by providing saplings with high commercial value. Furthermore, mineral nutrition plays an important role in the induction of organogenic responses. The objective of this study was to evaluate the effects of boron and calcium in the organogenesis of nodal segments from seedlings of Eucalyptus grandis growing under in vitro conditions. The concentration of boron and calcium in MS medium was modified to induce organogenic responses in 45-day-old nodal segments used as explants. After 60 days, the fresh weight, dry weight, ratio of fresh and dry weight, relative water content and relative matter content accumulated by the explants were evaluated. The concentrations of boron and calcium in the culture medium influenced the in vitro organogenic control of Eucalyptus grandis. Reduced combinations of boron and calcium induced callus formation and dry matter accumulation in the explants. A boron concentration of 100% (1.10 mg L-1) combined with 100% (119.950 mg L-1) and 200% (239.900 mg L-1) of calcium, and 200% (2.20 mg L-1) of boron combined with 100% (119.950 mg L-1) of calcium allowed the induction of well-developed buds, which can be used for the regeneration of micro-plants. |
| Idioma: | Inglés |