| Título: |
Micropropagation of an Eucalyptus hybrid (Eucalyptus benthamii x Eucalyptus dunnii) - doi: 10.4025/actasciagron.v33i4.8317 Micropropagation of an Eucalyptus hybrid (Eucalyptus benthamii x Eucalyptus dunnii) - doi: 10.4025/actasciagron.v33i4.8317 |
| Autores: |
Brondani, Gilvano Ebling; Universidade Federal do Paraná Dutra, Leonardo Ferreira; Empresa Brasileira de Pesquisa Agropecuária Wendling, Ivar; Empresa Brasileira de Pesquisa Agropecuária Grossi, Fernando; Universidade Federal do Paraná Hansel, Fabricio Augusto; Empresa Brasileira de Pesquisa Agropecuária Araujo, Marla Alessandra; Universidade Federal de Lavras |
| Fecha: | 2011-09-29 |
| Publicador: | Acta Scientiarum. Agronomy |
| Fuente: |
 |
| Tipo: |
Pesquisa |
| Tema: |
5.01.03.04-0 in vitro establishment; culture medium; cloning; BAP; NAA; GA3 Produção de Mudas in vitro establishment; culture medium; cloning; BAP; NAA; GA3 |
| Descripción: |
This study was designed to micropropagate E. benthamii x E. dunnii, by testing chlorine concentrations for explant asepsis, the optimal concentrations of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) for bud proliferation, and the ratio between BAP and gibberellic acid (GA3) in two nutrient media for shoot elongation. Nodal segments from H12, H19 and H20 clones were disinfected with 0.5, 1.0, 1.5 and 2.0% (v v-1) of chlorine. Explants were grown on ½MS medium supplemented with BAP (0, 0.25, 0.50, 0.75 and 1.00 mg L-1) and NAA (0, 0.025, 0.050, 0.075 and 0.100 mg L-1) for bud production. They were elongated on MS and ½MS media supplemented with BAP (0, 0.05 and 0.10 mg L-1) and GA3 (0, 0.1, 0.2 and 0.3 mg L-1). The 0.50 mg L-1 BAP and 0.050 mg L-1 NAA combination was optimal for bud proliferation for H12 and H20. GA3 concentrations of 0.10 and 0.2-1 mg L-1 combined with 0.10 mg L-1 BAP on ½MS resulted in the longest shoots, for H12 and H20, respectively. Regardless of clone, the rooting rate was low, with an average of 12.0% and 14.4% of plants having roots for in vitro and ex vitro conditions, respectively. This study was designed to micropropagate E. benthamii x E. dunnii, by testing chlorine concentrations for explant asepsis, the optimal concentrations of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) for bud proliferation, and the ratio between BAP and gibberellic acid (GA3) in two nutrient media for shoot elongation. Nodal segments from H12, H19 and H20 clones were disinfected with 0.5, 1.0, 1.5 and 2.0% (v v-1) of chlorine. Explants were grown on ½MS medium supplemented with BAP (0, 0.25, 0.50, 0.75 and 1.00 mg L-1) and NAA (0, 0.025, 0.050, 0.075 and 0.100 mg L-1) for bud production. They were elongated on MS and ½MS media supplemented with BAP (0, 0.05 and 0.10 mg L-1) and GA3 (0, 0.1, 0.2 and 0.3 mg L-1). The 0.50 mg L-1 BAP and 0.050 mg L-1 NAA combination was optimal for bud proliferation for H12 and H20. GA3 concentrations of 0.10 and 0.2-1 mg L-1 combined with 0.10 mg L-1 BAP on ½MS resulted in the longest shoots, for H12 and H20, respectively. Regardless of clone, the rooting rate was low, with an average of 12.0% and 14.4% of plants having roots for in vitro and ex vitro conditions, respectively. |
| Idioma: | Inglés |